PHARMACOLOGICAL RESPONSES OF THIAMINE-DEFICIENT RAT TISSUES

Studies on the physiological functions of thiamine. I. The effects of thiamine deficiency and thiamine antagonists on the oxidation of alpha-keto acids by rat tissues.

Although thiamine has been known and studied for many years, there is still much we do not know about its physiological functions and mechanism of action in biological systems, particularly in animal tissues. Earlier workers have shown that thiamine diphosphate (cocarboxylase) functions as a coenzyme in the metabolism of pyruvate and a-ketoglutarate (l-9). However, the question regarding the im...

Glyoxalase and methylglyoxal in thiamine-deficient rats.

Studies of thiamine deficient rat brains with in vivo and in vitro proton NMR spectroscopy

J t oduction T?I!amine, vitamin Bl, is essential for aerobic glucose metabolism. Thiamine deficiency (TD), a frequent complication of alcoholism caused by decreased thiamine absorption, may result in brain damage known as Wernicke’s encephalopathy. In chronic alcoholics, neurobehavioral deficits related to TD and brain glucose metabolism have been shown to recover during abstinence. However, th...

Effects of thiamine deficiency and thiamine administration on the thiamine diphosphate-dependent enzymes in rat liver.

The activities of pyruvate and 2-oxoglutarate dehydrogenases were determined by a spectrophotometric method according to Massey, that of transketolase deter mined by Racker's method. The values of enzyme activity as well as the thiamine contents in the preparations were compared with those of the animals whose deficiencies were restored by thiamine administration. The thiamine deprivation resul...

Energy and glucose pathways in thiamine deficient primary rat brain microvascular endothelial cells.

Thiamine deficiency (TD) results in lactate acidosis, which is associated with neurodegeneration. The aim of this study was to investigate this alteration in primary rat brain endothelia. Spectrophotometric analysis of culture media revealed that only a higher concentration of pyrithiamine, which accelerates the intracellular blocking of thiamine, significantly elevated the lactate level and la...